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人抗載脂蛋白抗體A1酶免試劑盒,(Apo A1)ELISA檢測試劑盒 ,英文名:Apo A1 ELISA Kit ,英文縮寫:Apo A1 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗載脂蛋白抗體A1ELISA檢測試劑盒,Apo A1 檢測試劑盒, Apo A1 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。人抗載脂蛋白抗體A1酶免試劑盒,(Apo A1)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預(yù)處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重
甲狀腺過氧化物酶(TPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thyroid Peroxidase (TPO)48T/96TY08435Mus musculus (Mouse)
血管活性腸肽(VIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vasoactive Intestinal Peptide (VIP)48T/96TY08436Homo sapiens (Human)
血管活性腸肽(VIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vasoactive Intestinal Peptide (VIP)48T/96TY08437Rattus norvegicus (Rat)
血管活性腸肽(VIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vasoactive Intestinal Peptide (VIP)48T/96TY08438Mus musculus (Mouse)
P物質(zhì)(SP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Substance P (SP)48T/96TY08439Homo sapiens (Human)
抗酒石酸酸性磷酸酶(ACP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acid Phosphatase 5, Tartrate Resistant (ACP5)48T/96TY08440Homo sapiens (Human)
抗酒石酸酸性磷酸酶(ACP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acid Phosphatase 5, Tartrate Resistant (ACP5)48T/96TY08441Rattus norvegicus (Rat)
抗酒石酸酸性磷酸酶(ACP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acid Phosphatase 5, Tartrate Resistant (ACP5)48T/96TY08442Mus musculus (Mouse)
脂蛋白脂酶(LIPD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipase, Lipoprotein (LIPD)48T/96TY08443Homo sapiens (Human)
載脂蛋白H(APOH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein H (APOH)48T/96TY08444Rattus norvegicus (Rat)
載脂蛋白H(APOH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein H (APOH)48T/96TY08445Mus musculus (Mouse)
C反應(yīng)蛋白(CRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C Reactive Protein (CRP)48T/96TY08446Homo sapiens (Human)
基質(zhì)細胞衍生因子4(SDF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Stromal Cell Derived Factor 4 (SDF4)48T/96TY08447Homo sapiens (Human)
基質(zhì)細胞衍生因子4(SDF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Stromal Cell Derived Factor 4 (SDF4)48T/96TY08448Rattus norvegicus (Rat)
基質(zhì)細胞衍生因子4(SDF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Stromal Cell Derived Factor 4 (SDF4)48T/96TY08449Mus musculus (Mouse)
周期素依賴性激酶抑制因子2A(CDKN2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)48T/96TY08450Homo sapiens (Human)
周期素依賴性激酶抑制因子2A(CDKN2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)48T/96TY08451Rattus norvegicus (Rat)
周期素依賴性激酶抑制因子2A(CDKN2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)48T/96TY08452Mus musculus (Mouse)
淀粉酶γ(Amyγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amylase Gamma (Amyg)48T/96TY08453Homo sapiens (Human)
唾液淀粉酶α1(AMY1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amylase Alpha 1, Salivary (AMY1)48T/96TY08454Rattus norvegicus (Rat)
組織因子通道抑制因子(TFPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Factor Pathway Inhibitor (TFPI)48T/96TY08455Mus musculus (Mouse)
組織因子通道抑制因子(TFPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Factor Pathway Inhibitor (TFPI)48T/96TY08456Oryctolagus cuniculus (Rabbit)
磷脂酶A2激活蛋白(PLAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2 Activating Protein (PLAA)48T/96TY08457Rattus norvegicus (Rat)
磷脂酶A2激活蛋白(PLAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2 Activating Protein (PLAA)48T/96TY08458Mus musculus (Mouse)
血紅素氧合酶1(HO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heme Oxygenase 1, Decycling (HO1)48T/96TY08459Homo sapiens (Human)
血紅素氧合酶1(HO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heme Oxygenase 1, Decycling (HO1)48T/96TY08460Rattus norvegicus (Rat)
血紅素氧合酶1(HO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heme Oxygenase 1, Decycling (HO1)48T/96TY08461Mus musculus (Mouse)
糖抗原125(CA125)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbohydrate Antigen 125 (CA125)48T/96TY08462Mus musculus (Mouse)
激肽釋放酶3(KLK3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 3 (KLK3)48T/96TY08463Rattus norvegicus (Rat)
激肽釋放酶3(KLK3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 3 (KLK3)48T/96TY08464Mus musculus (Mouse)
前列腺酸性磷酸酶(ACP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acid Phosphatase 3, Prostatic (ACP3)48T/96TY08465Rattus norvegicus (Rat)
前列腺酸性磷酸酶(ACP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acid Phosphatase 3, Prostatic (ACP3)48T/96TY08466Mus musculus (Mouse)
甲胎蛋白(αFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein (aFP)48T/96TY08467Rattus norvegicus (Rat)
甲胎蛋白(αFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein (aFP)48T/96TY08468Mus musculus (Mouse)
髓鞘堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myelin Basic Protein (MBP)48T/96TY08469Homo sapiens (Human)
髓鞘堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myelin Basic Protein (MBP)48T/96TY08470Rattus norvegicus (Rat)
髓鞘堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myelin Basic Protein (MBP)48T/96TY08471Mus musculus (Mouse)
細胞周期素D1(CCND1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin D1 (CCND1)48T/96TY08472Homo sapiens (Human)
細胞周期素D1(CCND1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin D1 (CCND1)48T/96TY08473Rattus norvegicus (Rat)
細胞周期素D1(CCND1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin D1 (CCND1)48T/96TY08474Mus musculus (Mouse)
細胞周期素D2(CCND2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin D2 (CCND2)48T/96TY08475Homo sapiens (Human)
細胞周期素D2(CCND2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin D2 (CCND2)48T/96TY08476Rattus norvegicus (Rat)
細胞周期素D2(CCND2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin D2 (CCND2)48T/96TY08477Mus musculus (Mouse)
細胞周期素D3(CCND3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin D3 (CCND3)48T/96TY08478Homo sapiens (Human)
細胞周期素D3(CCND3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin D3 (CCND3)48T/96TY08479Rattus norvegicus (Rat)
細胞周期素D3(CCND3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin D3 (CCND3)48T/96TY08480Mus musculus (Mouse)
熱休克蛋白β2(HSPβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein Beta 2 (HSPb2)48T/96TY08481Homo sapiens (Human)
熱休克蛋白40(HSP40)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 40 (HSP40)48T/96T Y08482Homo sapiens (Human)
熱休克蛋白90(HSP90)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 90 (HSP90)48T/96TY08483Homo sapiens (Human)
淀粉樣蛋白β1-40(Aβ1-40)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amyloid Beta Peptide 1-40 (Ab1-40)48T/96TY08484Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。