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小鼠抗透明帶抗體酶免試劑盒,(aZP)ELISA檢測試劑盒 ,英文名:aZP ELISA Kit ,英文縮寫:aZP ,常見試劑盒規格:96T/48T,產品別名:小鼠抗透明帶抗體ELISA檢測試劑盒,aZP 檢測試劑盒, aZP 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠抗透明帶抗體酶免試劑盒,(aZP)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
刺猬因子相互作用蛋白(HHIP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hedgehog Interacting Protein (HHIP)48T/96TY13085Mus musculus (Mouse)
微纖絲關聯蛋白4(MFAP4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Microfibrillar Associated Protein 4 (MFAP4)48T/96TY13086Rattus norvegicus (Rat)
左右決定因子1(LEFTY1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Left/Right Determination Factor 1 (LEFTY1)48T/96TY13087Homo sapiens (Human)
轉膠蛋白(TAGLN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transgelin (TAGLN)48T/96TY13088Rattus norvegicus (Rat)
過氧化氫酶(CAT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Catalase (CAT)48T/96TY13089Mus musculus (Mouse)
細胞色素P450氧化還原酶(CPR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cytochrome P450 Reductase (CPR)48T/96TY13090Homo sapiens (Human)
弗林蛋白酶(FUR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Furin (FUR)48T/96TY13091Homo sapiens (Human)
微精蛋白β(MSMβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Microseminoprotein Beta (MSMb)48T/96TY13092Homo sapiens (Human)
銅轉運蛋白1(COPT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Copper Transporter 1 (COPT1)48T/96TY13093Homo sapiens (Human)
維生素D受體(VDR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vitamin D Receptor (VDR)48T/96TY13094Mus musculus (Mouse)
微粒體甘油三酸酯轉運蛋白(MTTP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Microsomal Triglyceride Transfer Protein (MTTP)48T/96TY13095Mus musculus (Mouse)
溶血磷脂酸受體1(LPAR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lysophosphatidic Acid Receptor 1 (LPAR1)48T/96TY13096Homo sapiens (Human)
圍脂滴蛋白4(PLIN4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Perilipin 4 (PLIN4)48T/96TY13097Mus musculus (Mouse)
含卷曲螺旋域蛋白80(CCDC80)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Coiled Coil Domain Containing Protein 80 (CCDC80)48T/96TY13098Homo sapiens (Human)
熱休克蛋白47(HSP47)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heat Shock Protein 47 (HSP47)48T/96TY13099Homo sapiens (Human)
核纖層蛋白B1(LMNB1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lamin B1 (LMNB1)48T/96TY13100Homo sapiens (Human)
戴帽蛋白肌Z系β(CAPZβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Capping Protein Muscle Z Line Beta (CAPZb)48T/96TY13101Homo sapiens (Human)
高爾基體蛋白A8家族成員A(GOLGA8A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgin A8 Family, Member A (GOLGA8A)48T/96TY13102Homo sapiens (Human)
成纖維細胞生長因子5(FGF5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 5 (FGF5)48T/96TY13103Homo sapiens (Human)
嵌套蛋白(NES)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nestin (NES)48T/96TY13104Homo sapiens (Human)
碳酸酐酶Ⅵ(CA6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase VI (CA6)48T/96TY13105Homo sapiens (Human)
透明質酸合酶2(HAS2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hyaluronan Synthase 2 (HAS2)48T/96TY13106Homo sapiens (Human)
脯氨酸/精氨酸豐富端亮氨酸豐富重復蛋白(PRELP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP)48T/96TY13107Homo sapiens (Human)
絲聚蛋白(FLG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Filaggrin (FLG)48T/96TY13108Homo sapiens (Human)
血小板反應蛋白解整合素金屬肽酶4(ADAMTS4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)48T/96TY13109Homo sapiens (Human)
肌營養不良蛋白關聯糖蛋白1(DAG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dystrophin Associated Glycoprotein 1 (DAG1)48T/96TY13110Mus musculus (Mouse)
δ樣蛋白4(δLL4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Delta Like Protein 4 (dLL4)48T/96TY13111Homo sapiens (Human)
含CUB域蛋白1(CDCP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for CUB Domain Containing Protein 1 (CDCP1)48T/96TY13112Homo sapiens (Human)
Smad同源物3(Smad3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 3 (Smad3)48T/96TY13113Rattus norvegicus (Rat)
補體因子H相關蛋白3(CFHR3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Complement Factor H Related Protein 3 (CFHR3)48T/96TY13114Homo sapiens (Human)
角蛋白4(KRT4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Keratin 4 (KRT4)48T/96TY13115Homo sapiens (Human)
ⅩⅣ型膠原(COL14)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type XIV (COL14)48T/96TY13116Homo sapiens (Human)
雙特異性酪氨酸磷酸化調節激酶1A(DYRK1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A)48T/96TY13117Mus musculus (Mouse)
剪切多聚腺苷酸化特異性因子1(CPSF1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1)48T/96TY13118Homo sapiens (Human)
Enah/Vasp樣蛋白(EVL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Enah/Vasp Like Protein (EVL)48T/96TY13119Homo sapiens (Human)
趨化因子C-X-C-基元受體4(CXCR4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chemokine C-X-C-Motif Receptor 4 (CXCR4)48T/96TY13120Homo sapiens (Human)
含V-Set域T細胞激活抑制因子1(VTCN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1)48T/96TY13121Homo sapiens (Human)
不均一核核糖核蛋白U(HNRPU)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heterogeneous Nuclear Ribonucleoprotein U (HNRPU)48T/96TY13122Homo sapiens (Human)
前折疊蛋白亞基1(PFDN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prefoldin Subunit 1 (PFDN1)48T/96TY13123Homo sapiens (Human)
神經元衍生孤兒受體1()檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neuron Derived Orphan Receptor 1 ()48T/96TY13124Rattus norvegicus (Rat)
蝰蛇毒素(Viperin)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Viperin48T/96TY13125Homo sapiens (Human)
亨廷頓蛋白(HTT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Huntingtin (HTT)48T/96TY13126Mus musculus (Mouse)
亨廷頓蛋白(HTT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Huntingtin (HTT)48T/96TY13127Rattus norvegicus (Rat)
信號素3F(SEMA3F)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Semaphorin 3F (SEMA3F)48T/96TY13128Homo sapiens (Human)
血管內皮鈣黏蛋白(CDH5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cadherin 5 (CDH5)48T/96TY13129Mus musculus (Mouse)
β-位淀粉樣前體蛋白裂解酶1(βACE1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Beta-Site APP Cleaving Enzyme 1 (bACE1)48T/96TY13130Mus musculus (Mouse)
谷胱甘肽S轉移酶π1(GSTπ1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Pi (GSTp)48T/96TY13131Sus scrofa; Porcine (Pig)
90kDa熱休克蛋白αA1(HSP90αA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heat Shock Protein 90kDa Alpha A1 (HSP90aA1)48T/96TY13132Mus musculus (Mouse)
Apelin受體(APLNR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apelin Receptor (APLNR)48T/96TY13133Rattus norvegicus (Rat)
骨成型蛋白受體1B(BMPR1B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Bone Morphogenetic Protein Receptor 1B (BMPR1B)48T/96TY13134Homo sapiens (Human)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。