服務熱線
021-54479081
021-54461587
產品展示PRODUCTS
本產品僅用于科研,不用于臨床
小鼠可溶性CD30酶免試劑盒,(sCD30)ELISA檢測試劑盒 ,英文名:sCD30 ELISA Kit ,英文縮寫:sCD30 ,常見試劑盒規格:96T/48T,產品別名:小鼠可溶性CD30ELISA檢測試劑盒,sCD30 檢測試劑盒, sCD30 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠可溶性CD30酶免試劑盒,(sCD30)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
白介素28A(IL28A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 28A (IL28A)48T/96TY14034Mus musculus (Mouse)
生長分化因子2(GDF2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 2 (GDF2)48T/96TY14035Rattus norvegicus (Rat)
白介素17B(IL17B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 17B (IL17B)48T/96TY14036Mus musculus (Mouse)
成纖維細胞生長因子10(FGF10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 10 (FGF10)48T/96TY14037Mus musculus (Mouse)
表皮調節素(EREG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Epiregulin (EREG)48T/96TY14038Mus musculus (Mouse)
表皮調節素(EREG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Epiregulin (EREG)48T/96TY14039Rattus norvegicus (Rat)
趨化因子C-C-基元受體1(CCR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Receptor 1 (CCR1)48T/96TY14040Mus musculus (Mouse)
肝X受體α(LXRα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Liver X Receptor Alpha (LXRa)48T/96TY14041Mus musculus (Mouse)
翻譯調節腫瘤蛋白1(TPT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Protein, Translationally Controlled 1 (TPT1)48T/96TY14042Homo sapiens (Human)
成纖維細胞生長因子9(FGF9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 9 (FGF9)48T/96TY14043Bos taurus; Bovine (Cattle)
纖維膠凝蛋白1(FCN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ficolin 1 (FCN1)48T/96TY14044Rattus norvegicus (Rat)
整合素β6(ITGβ6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Integrin Beta 6 (ITGb6)48T/96TY14045Homo sapiens (Human)
核糖核酸酶A(RNASE1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ribonuclease A (RNASE1)48T/96TY14046Bos taurus; Bovine (Cattle)
脂肪酸合酶(FASN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Synthase (FASN)48T/96TY14047Chicken (Gallus)
汗酸酶αL(IDUα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Iduronidase Alpha L (IDUa)48T/96TY14048Homo sapiens (Human)
促凋亡蛋白1(APOPT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apoptogenic 1 (APOPT1)48T/96TY14049Homo sapiens (Human)
巖藻糖變旋酶(FucM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fucose Mutarotase (FucM)48T/96TY14050Homo sapiens (Human)
Nesprin 4蛋白(Nesp4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nesprin 4 (Nesp4)48T/96TY14051Homo sapiens (Human)
著絲粒蛋白32(CENP32)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Centromere Protein 32 (CENP32)48T/96TY14052Homo sapiens (Human)
癌/睪丸抗原62(CTAG62)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cancer/Testis Antigen 62 (CTAG62)48T/96TY14053Homo sapiens (Human)
Sugen激酶071(SGK071)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sugen Kinase 071 (SGK071)48T/96TY14054Homo sapiens (Human)
Ashwin蛋白(Ash)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ashwin (Ash)48T/96TY14055Homo sapiens (Human)
蛋白C10(C10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein C10 (C10)48T/96TY14056Homo sapiens (Human)
B-黑色素瘤抗原5(BAGE5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for B-Melanoma Antigen 5 (BAGE5)48T/96TY14057Homo sapiens (Human)
著絲粒蛋白36(CENP36)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Centromere Protein 36 (CENP36)48T/96TY14058Homo sapiens (Human)
可溶性半乳糖苷結合凝集素16(LGALS16)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lectin, Galactoside-Binding Soluble 16 (LGALS16)48T/96TY14059Homo sapiens (Human)
內切核酸酶V(ENDOV)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endonuclease V (ENDOV)48T/96TY14060Homo sapiens (Human)
Kazrin蛋白(KAZN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kazrin (KAZN)48T/96TY14061Homo sapiens (Human)
前庭蛋白1(VEST1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vestibule 1 (VEST1)48T/96TY14062Homo sapiens (Human)
殺傷蛋白(KLLN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Killin (KLLN)48T/96TY14063Homo sapiens (Human)
胰蛋白酶X3(TRYX3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Trypsin X3 (TRYX3)48T/96TY14064Homo sapiens (Human)
Whirlin蛋白(WHRN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Whirlin (WHRN)48T/96TY14065Homo sapiens (Human)
皮卡丘素(Pikachurin)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Pikachurin48T/96TY14066Homo sapiens (Human)
血色病蛋白(HFE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hemochromatosis (HFE)48T/96TY14067Homo sapiens (Human)
微腦磷脂1(MCPH1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Microcephalin 1 (MCPH1)48T/96TY14068Homo sapiens (Human)
腎病型胱胺酸癥蛋白(CTNS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cystinosis, Nephropathic (CTNS)48T/96TY14069Homo sapiens (Human)
腦蛋白44(BRP44)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Brain Protein 44 (BRP44)48T/96TY14070Homo sapiens (Human)
Hairless蛋白(HR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hairless (HR)48T/96TY14071Homo sapiens (Human)
載脂蛋白L6(APOL6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apolipoprotein L6 (APOL6)48T/96TY14072Homo sapiens (Human)
Atonal同源物1(ATOH1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Atonal Homolog 1 (ATOH1)48T/96TY14073Homo sapiens (Human)
鈣黏蛋白12(CDH12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cadherin 12 (CDH12)48T/96TY14074Homo sapiens (Human)
矢車菊苷γ3(CENTγ3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Centaurin Gamma 3 (CENTg3)48T/96TY14075Homo sapiens (Human)
矢車菊苷δ3(CENTδ3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Centaurin Delta 3 (CENTd3)48T/96TY14076Homo sapiens (Human)
矢車菊苷δ2(CENTδ2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Centaurin Delta 2 (CENTd2)48T/96TY14077Homo sapiens (Human)
矢車菊苷δ1(CENTδ1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Centaurin Delta 1 (CENTd1)48T/96TY14078Homo sapiens (Human)
羧肽酶X1(CPX1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxypeptidase X1 (CPX1)48T/96TY14079Homo sapiens (Human)
二羥基激酶2(DAK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dihydroxyacetone Kinase 2 (DAK)48T/96TY14080Homo sapiens (Human)
加帽酶2(DCP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Decapping Enzyme 2 (DCP2)48T/96TY14081Homo sapiens (Human)
防御素β118(DEFβ118)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Defensin Beta 118 (DEFb118)48T/96TY14082Homo sapiens (Human)
Derlin 1蛋白(DERL1)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Derlin 1 (DERL1)48T/96TY14083Homo sapiens (Human)
Derlin 2蛋白(DERL2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Derlin 2 (DERL2)48T/96TY14084Homo sapiens (Human)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。